How to Use GNPS in 10 Minutes | Molecular Networking Step-by-Step

Learn how to perform **Molecular Networking on GNPS in just 10 minutes**! In this step-by-step tutorial, I show you how to upload your MGF file from MZmine, run the GNPS Molecular Networking workflow, understand the processing steps, and download the GraphML file for Cytoscape visualization. If you’ve already prepared your data in MZmine and exported your MGF file, this video will guide you through the entire GNPS workflow — from job submission to downloading your molecular network results. 🔬 In this video, you will learn: • How to create and sign into a GNPS account • How to upload MGF spectrum files • How to submit a Molecular Networking job • What GNPS does during spectral clustering and library matching • How to interpret the GNPS results page • Where to download GraphML files for Cytoscape • How to prepare your data for network visualization This tutorial is perfect for: ✔ Metabolomics researchers ✔ LC-MS / MS/MS users ✔ Natural products scientists ✔ PhD and graduate students ✔ Anyone using MZmine + GNPS for Feature-Based Molecular Networking (FBMN) By the end of this video, you will have successfully generated your molecular network and downloaded the GraphML file ready for Cytoscape visualization. In the next video, we will open the GraphML file in Cytoscape and create a publication-ready molecular network visualization. Keywords: GNPS tutorial, molecular networking GNPS, Feature-Based Molecular Networking, FBMN workflow, MGF upload GNPS, LC-MS data analysis, MS/MS networking, Cytoscape GraphML download, metabolomics molecular networking #GNPS #MolecularNetworking #FBMN #Metabolomics #LCMS #MassSpectrometry #Cytoscape #MZmine