Cytek Aurora Training Part 2: Reference Controls

Aurora User Training Spectral Flow Cytometry PART 2 - Reference Controls 0:00 Intro 0:44 Overview of rules for reference controls 1:25 Rule 1 sufficient number of events 5:48 Rule 2 clear separation of positive 7:03 Rule 3 matching autofluorescence 10:09 Rule 4 fluorophore brightness 12:22 Rule 5 fluorophore signature 17:12 Quiz 20:29 Tips for manufacturing controls 22:22 Should you use beads or cells? For more information on when you can reuse old controls, see this video:    • How to combine multiple aurora experiments...   Advanced tips for setting up the universal negative feature in SpectroFlo:    • Advanced Tips for Organizing Unstained con...   Tutorial on determining if beads or cells are best to unmix:    • Advanced Unmixing in SpectroFlo (Cytek Aur...   This is a comprehensive course for researchers who would like to use the Cytek Aurora. This course assumes that researchers are already familiar with conventional flow cytometry. Slides can be downloaded here: https://drive.google.com/file/d/1wF9-... More information and resources can be found here: https://voices.uchicago.edu/ucflow/sp... View the full training course:    • Cytek Aurora Training Course   Cytometry and Antibody Technology Facility University of Chicago I have no affiliation with Cytek Biosciences.