Lecture On Master Bilirubin Metabolism & Jaundice Types in 25 Minutes | Medical Biochemistry | MBBS

Welcome to this comprehensive Biochemistry lecture for the MBBS (2025-2026 Batch) at Santiniketan Medical College & Hospital! 🩺 In this class, we dive deep into the clinical estimation of Bilirubin using the Diazo Method, understanding Jaundice, and breaking down Bilirubin metabolism from production to excretion. Whether you are an MBBS student preparing for exams, a biochemistry major, or a medical laboratory technician, this video will give you a crystal-clear understanding of Total, Direct, and Indirect Bilirubin estimation, calculation factors, and clinical interpretations. ⏳ Video Chapters (Click to navigate): [00:00] - Intro: Bilirubin Production & Conjugation in the Liver [01:25:00] - The Role of Bile Salts & Pigments [02:12:00] - Normal Reference Ranges for Bilirubin (Total & Direct) [03:13:00] - Jaundice: Types, Causes, Signs & Symptoms [06:01:00] - Principle of the Diazo Method for Bilirubin Estimation [08:38:00] - Direct vs. Indirect Bilirubin & The Role of Surfactants (DMSO) [11:03:00] - Understanding the Reagents (R1, R2, R3) [13:35:00] - Procedure for Estimation using a Semi-Auto Analyzer [14:27:00] - Calculation Using Factors (21 for Total, 12 for Direct) [19:26:00] - Clinical Interpretation: Hyperbilirubinemia & Hypobilirubinemia [21:51:00] - Sample Interferences (Hemolyzed & Lipemic Samples) [23:04:00] - Quick Lecture Summary & Review #BilirubinEstimation #Biochemistry #MBBS #Jaundice #DiazoMethod #MedicalStudent #BiochemistryLectures #ClinicalPathology #SantiniketanMedicalCollege #PathologyLab SANTINIKETAN MEDICAL COLLEGE & HOSPITAL Real Classroom Demonstration/Lecture On: BIOCHEMISTRY by Dr. Prajna Sarkar MBBS Batch: (2025-26) Conducted on: 09-06-26