Illinois State Univ. SP8 confocal microscope overview and applications (2020). Part 1 of 3.
Oct 2020. Kevin Edwards discusses the Illinois State University (Normal, Illinois) confocal microscopy core facility and presents several applications that may be of interest to external users across biology. Part 1. Basic overview of the Leica SP8 Confocal system; lightpath; White Light Laser; Synergy among components. 15 min. See also... Part 2: • Illinois State Univ. SP8 confocal microsco... Spectral fingerprinting with Ex/Em plots; Fluorescence Lifetime Imaging Microscopy (FLIM). 13 min. Part 3. • Illinois State Univ. SP8 confocal microsco... Lightning deconvolution with an example using endosomes; use of Zoom conferencing on the confocal. 18 min. Part 4. • Illinois State University Confocal Microsc... This provides an overview of the SP8 confocal lightpath and covers details of the excitation side: White light laser (WLL) mechanism; AOTF control of WLL; The laser array; Scanner options.
Illinois State Univ. SP8 confocal microscope overview and applications (2020). Part 2 of 3.
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