Intro to Pixel Classification in ilastik - AskErinDearBeth Episode 11

In this episode, Erin and Beth use cells imaged with phase contrast imaging to introduce pixel classification in ilastik. Beth also brings in a set of multi-color synthetic cell images to introduce the concept of overfitting. She shows the uncertainty view and makes ~*magic*~ happen with just a few pixels! For a deep dive into why we ~*demand*~ you use the smallest brush when using ilastik, check out Episode 13    • How to Train a Pixel Classifier - AskErinD...   #science #openscience #microscopy #tutorial #opensource #biology #imageanalysis #segmentation #cellprofiler #ilastik ================================================== On Ask Erin/Dear Beth, bioimage analysis experts Beth Cimini and Erin Weisbart, of the Imaging Platform at the Broad Institute of MIT and Harvard, answer your image analysis questions! Whether it’s about tuning or troubleshooting, segmenting or measuring, Beth and Erin have opinions and by golly are they going to share them! Using mostly CellProfiler, but with expertise in many other image analysis softwares such as ImageJ/FIJI, Cellpose, Piximi, and more, no image analysis question is too hard, so ask away! Submit your own questions at broad.io/AskErinDearBeth Images and pipelines we’ve used are available for you to download and play with at https://ciminilab.github.io/AskErin_D...