NGS Explained: Next Generation Sequencing Step-by-Step
NGS (Next Generation Sequencing) can sequence a whole human genome in a single day, a process that took over 30 years with the Human Genome Project. This animation explains how Illumina sequencing and NGS work step-by-step, including library preparation, cluster generation, sequencing by synthesis, and how billions of DNA strands are sequenced simultaneously. You'll also learn how NGS compares to Sanger sequencing, how sequencing reads are filtered and mapped to a reference genome, and the key applications of NGS in research and medicine. Correction: 0:13:00 should read that 85% of the human genome was completed by 2000, not 2023. By 2023, 92% of the human genome was completed. 📖 Read the blog post → https://clevalab.com/post/a-step-by-s... 🌏 https://clevalab.com 👉 Watch next: "qPCR and PCR Explained" →    • qPCR and PCR Explained: Real-Time PCR Anim...  ✉️ Join the ClevaLab Community for free guides and updates on the upcoming courses → https://www.clevalab.com/email-subscr... SOCIAL MEDIA LINKS Twitter:   / _clevalab  Instagram:   / _clevalab  Pinterest: https://www.pinterest.com.au/ClevaLab Tiktok:   / clevalab  Facebook:   / clevalab  CHAPTERS: 00:00 From the Human Genome Project to NGS 00:39 NGS vs Sanger Sequencing 01:01 The Basic Principle of NGS 01:23 DNA and RNA Purification and QC 01:38 Library Preparation - The First Step of NGS 02:32 Sequencing by Synthesis and The Sequencing Reaction 03:19 Cluster Generation From the Library Fragment 03:59 Sequencing of the Forward Strand 04:42 The First Index is Read 04:55 The Second Index is Read 05:15 Sequencing of the Reverse Strand 05:25 Filtering and Mapping of the Reads 05:47 Demultiplexing and Mapping to the Reference 06:19 What is Read Depth in NGS? 06:49 How is NGS being used? 07:01 What Types of NGS Applications Are There? #ngs #sequencing #ClevaLab
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