Confocal Microscopy

Animated video on Confocal Microscopy. The confocal microscope is a modified fluorescence microscope, that uses LASER insted of mercury arch lamp.Confocal microscopy also termed as confocal laser scanning microscopy (CLSM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation.Confocal microscopy is widely used for optical sectioning and reconstruction of 3D image of specimen.