Determination of Minimum Inhibitory Concentration (MIC)

Welcome to our suite of NUI Galway Microbiology teaching videos. We hope that they will guide you in performing your experiments in a scientific and safe manner. In this video we demonstrate how to determine the minimum inhibitory concentration (MIC) for an antibiotic called nalidixic acid. MIC is the lowest concentration of an antibiotic that inhibits growth of a bacterial strain. MICs are typically used for antimicrobial susceptibility testing and can be carried out for multiple antibiotics. In a clinical setting, a 96 well plate is typically used. For the purposes of demonstrating the method, a 12 well plate was chosen for this video.