A basic overview of Immunohistochemistry (IHC) in free-floating tissue

Note: This seminar series has permanently moved to the EMGSS YouTube channel. For more videos in this series, visit:    / @emgssmcgilluniversity9527   This technical seminar was presented by Morgan Foret, a PhD candidate in the Cuello lab (McIntyre Medical Building) with help from Ryan Rys, a PhD candidate in the Johnson lab (Lady Davis Institute for Medical Research) on March 5th, 2021. This seminar is part of a biomedical research technical seminar series hosted by McGill University's Experimental Medicine Graduate Students' Society (EMGSS), Graduate Association of Physiology (GAP) and Graduate Association of Pharmacology and Therapeutics Students (GAPTS). See emgss.org/tss for more. This seminar covered the basics of how to perform immunohistochemistry (IHC) for both brightfield and fluorescent labelling in free-floating brain tissue sections and briefly in other tissue types. Topics included how to select and troubleshoot primary and secondary antibodies as well as other experimental conditions to consider depending on the tissue and protein of interest.