Marcadores moleculares basados en PCR: Marcadores SSR. Microsatelites | 26/95 | UPV

Title: PCR-Based Molecular Markers: SSR Markers. Microsatellites Description: This video describes the basis, methodology, and applications of microsatellite-type PCR-based molecular markers. Picó Sirvent, MB. (2012). PCR-Based Molecular Markers: SSR Markers. Microsatellites. http://hdl.handle.net/10251/16567 Automatic description: In this video, the expert explains the methodology for identifying SSR (Simple Sequence Repeats) markers or microsatellites in DNA sequences. Microsatellites consist of tandem repeats of basic nucleotide units, which are hypervariable and can be amplified and separated by length using PCR, revealing a polymorphic banding pattern that varies between individuals. Primers complementary to unique flanking sequences are used to ensure the specificity of each locus. Microsatellites vary in length due to polymerase slippage during replication and unequal crossing-over. This variability makes them useful multi-allelic markers for genetic studies. They are visualized by agarose or acrylamide gel electrophoresis, and acrylamide gels are preferred when alleles vary by a few bases. The expert highlights the usefulness of microsatellites for genetic variability studies and mapping due to their codominant and multi-allelic nature. The initial limitation of requiring the known sequence of the flanking regions is being overcome with massive sequencing. Although multi-loci analysis is more expensive than other methods, multiplex techniques and fluorochromes can be used to make the process more cost-effective and automate genotyping. Despite the difficulty of automation compared to modern markers such as SNPs, microsatellites remain widely used in genetic research. Author: Picó Sirvent María Belén Course: This video is 23/92 of the Plant Genetics and Breeding course.    • Genética y mejora genética vegetal   Course: This video is 26/95 of the MOOC Introduction to Plant Genetics.    • MOOC introducción a la genética vegetal   Polytechnic University of Valencia (UPV): https://www.upv.es More videos at:    / valenciaupv   Access our MOOCs: https://upvx.es #Molecular markers #PCR #Microsatellites #Genetic diversity #GENETICS