Buyer Beware - A Guide for Selecting Antibodies and Using Them Effectively for your Research

Per the name of this video, hopefully my advice will cut down on the issues you will have in trying to find the right antibody for your research goals. You can find more IHC advice in my manual, via my website. https://sites.google.com/view/kru-neu... Video parts: 0:00 - Intro 1:51 - How do antibodies made by a vendor bind to a very specific target? 4:55 - How are antibodies specific to the protein of one species and yet not target the same protein in a different species? (Demo of BLAST) 20:36 - Why does it matter what “host” the antibody comes from? 22:20 - Do you know the difference between monoclonal vs polyclonal antibodies? 30:00 - What information should be on a vendor's page to help inform us about whether the antibody will or will not work for our specific lab’s goals? 38:02 - If a vendor states that an antibody is approved for use in IHC but does not provide a photo of IHC staining, should you trust their recommendation? 40:15 - Do you know the difference between cell culture photos versus tissue section photos on a vendor website? 44:22 - Is your target localized to specific cell compartments, or not? 49:21 - Should I perform antigen retrieval if the vendor suggests it? 52:36 - If a company that we buy antibodies from suggests that we use a 1 in 100 dilution (stock to diluent), should we trust that suggestion? Why or why not? 54:52 - When a secondary antibody product is listed as “cross-adsorbed”, what might that mean, and why is it important? 59:39 - Is there a collective database that has info on most antibody products available, rather than just doing normal web searches? 1:02:38 - Where can one find cheaper antibodies? 1:11:45 - End