Protein Purification

Protein Purification aims to isolate a single type of protein from a biological tissue or culture. This video explains the most common methods applied to purify proteins, which are: Precipitation methods, filtration methods, electrophoresis and chromatographic methods. It is important to have an overview of these techniques in order to choose which method/methods suit the project best. Factors that can influence such a decision are: the desired level of purity, the accessibility of required equipment and the characteristics of the target protein. In practice multiple purification techniques are often combined. Time Code: 00:00 – Intro 00:20 – Content 00:49 – What is protein purification? 02:20 – Separate your protein from a biological tissue 02:53 – Choosing the right purification techniques 03:40 – Precipitation methods 03:58 – Salting out 05:10 – Chromatography 06:08 – Affinity chromatography 07:25 – Ion Exchange Chromatography 08:07 – Hydrophobic Interaction Chromatography 09:08 – Size Exclusion Chromatography 10:20 – Filtration Methods (Dialysis) 11:12 – Electrophoresis 12:08 – Native PAGE 13:29 – Outro At Cube Biotech we are experts in the purification of protein with over 40 years of experience. We offer a wide range of protein purification products: https://cube-biotech.com/products/pro... Furthermore, we offer custom protein purification: https://cube-biotech.com/protein-serv... For general inquiries about our company check out: https://cube-biotech.com/ For more detailed information about protein purification techniques check out: Liu, S., Li, Z., Yu, B., Wang, S., Shen, Y., & Cong, H. (2020). Recent advances on protein separation and purification methods. Advances in colloid and interface science, 284, 102254. https://doi.org/10.1016/j.cis.2020.10...