Identifying Speckles in CellProfiler - AskErinDearBeth Episode 7

In this episode, Erin and Beth talk about speckles (a.k.a. small punctae a.k.a. spots a.k.a. sparkles). Beth leads us through several different ways to identify speckles in CellProfiler and explains why (or why not) to use each method. There are lots of biological reasons for identifying speckles/punctae/sparkles including telomeres (a use case for which Beth has a bunch of experience) and in situ sequencing (ISS) (a use case for which Erin has a bunch of experience). #science #openscience #microscopy #tutorial #opensource #biology #imageanalysis #segmentation #cellprofiler ================================================== On Ask Erin/Dear Beth, bioimage analysis experts Beth Cimini and Erin Weisbart, of the Imaging Platform at the Broad Institute of MIT and Harvard, answer your image analysis questions! Whether it’s about tuning or troubleshooting, segmenting or measuring, Beth and Erin have opinions and by golly are they going to share them! Using mostly CellProfiler, but with expertise in many other image analysis softwares such as ImageJ/FIJI, Cellpose, Piximi, and more, no image analysis question is too hard, so ask away! Submit your own questions at broad.io/AskErinDearBeth Images and pipelines we’ve used are available for you to download and play with at https://ciminilab.github.io/AskErin_D...