Cómo se procesa una ELISA de punto de corte? Helicobacter pylori IgM

Diagnosis of Helicobacter pylori infection can be achieved through invasive and noninvasive tests. Noninvasive tests include serological tests, which are based on the detection of specific antibodies against antigens of this bacterium, whether IgG, IgA, or IgM. This video shows the procedure for a micro ELISA (Mexlab brand kit, read on a Stat Fax 4700) for the detection of IgM antibodies to Helicobacter pylori. Although reagent and sample volumes may vary between kit brands, the procedure sequence is always the same regardless of the type of antibody and the microorganism being diagnosed: Sample pretreatment (in this case, serum dilution) Incubation of the diluted sample in the microwell Washing and addition of enzyme conjugate Washing and addition of TMB substrate Stopping the reaction with stop solution Reading absorbances and calculating results. Unlike quantitative ELISAs, these types of ELISAs for infectious serology are calibrated with only one standard. Results are expressed in ELISA units (EU) or index, which express the relative amount above or below a cutoff point obtained using a standard, with 1.1 EU or greater being a positive result. Disclaimers: The reagents and equipment shown in this or any video do not reflect the channel's preference or recommendation for any specific brand. It is the viewer's responsibility to verify the technique and calibrator values against the insert for the brand being used, as volumes and incubation times may vary between brands. We invite you to learn about our available clinical laboratory courses: Clinical Q., Hematology, and Urinalysis: https://www.udemy.com/course/laborato... Immunology and Hormones: https://www.udemy.com/course/laborato... Quality Control in the Clinical Laboratory: https://www.udemy.com/course/laborato... Hemostasis and Immunohematology: https://www.udemy.com/course/laborato...