Educator's Guide to Nanopore—16s: DNA extraction

DNA extraction: Column-based DNA purification using a Norgen microbiome kit. 00:00 Introduction 03:00 Sample lysis 14:54 Transfer and addition of binding buffers 25:14 Adding sample to columns 34:02 Washing columns 37:34 Drying columns and elution This guide is an educator-focused introduction to Nanopore DNA sequencing. Its primary goal is to share resources and approaches that make it easier to use Nanopore in the classroom, especially (but not exclusively) for course-based undergraduate research experiences (CUREs). In a series of videos, we will explain the basics of Nanopore sequencing as we share demonstration experiments. Full protocol available at: https://nanopore4edu.org/latest/annot...