Semina per inclusione con diluizioni seriali - Corso di microbiologia

Download material: https://iissgalileilab.blogspot.com/ With the help of 3D, we prepared this short video, so I thank Lorenzo and his classmates for their help. As I've already said, I prefer for the students to master the basics of the technique and be able to perform it fairly naturally, before introducing a whole series of details and "secondary" concepts. These are certainly essential for a correct analysis, but they create confusion and constitute excess material to assimilate. Once learned, there will be plenty of time to smooth out the rough edges. The convenience of using lactic acid bacteria as a sample lies both in their safety and in the fact that the dosage is often indicated on the packaging. The ones I used were 10 billion CFU/ml, which is quite high. To have the students start with a solution of 100,000 CFU/ml, I therefore had to dilute it before analysis. Obviously, for convenience and time reasons, I didn't pay much attention during the dilution. For this reason, and because we didn't pay much attention to maintaining sterility during the analysis, the result will certainly only be indicative. I repeat, this isn't a problem; on the contrary, the priority will be to acquire the technique and, subsequently, learn how to read and process the data. Furthermore, it will give me the opportunity to have the students reflect on the result: was it successful? Is it what we expected? If not, what errors might have been made? What should we have done to avoid them? In which steps were we more superficial? The students need to learn to ask questions and find answers. This is essential when working in a laboratory.