Practical Aspects on HPLC Method Development and LC Troubleshooting

Depending on the sample and the separation goals the selection of the column chemistry, column physics and the best suitable mobile phase conditions for the specific analytes and the individual needs is a key aspect for successful method development. In addition, time and productivity becomes more and more important. It is crucial to avoid trouble and find the most cost-efficient method to get precise and sensitive results. This seminar gives an overview about the available column base materials for HPLC and UHPLC, their benefits and limitations and the column chemistries for separation across compound polarities.