HPLC Demystified Part 3 | How to Maintain and Troubleshoot Your HPLC Like a Pro

🎁Download our HPLC Maintenance & Troubleshooting Checklist → https://zfrmz.com/fjn7iuq3bsK0mnFJyqFu 🔧 HPLC Demystified Part 3: How to Maintain and Troubleshoot Your HPLC Like a Pro Chromatography giving you headaches? Part 3 of the HPLC Demystified series is your lab-side guide to troubleshooting and routine maintenance — explained clearly by Dr. Lee Polite. Whether you're dealing with air bubbles, leaky seals, noisy baselines, or a worn-out rotor seal, this video walks you through the why, the how, and the fix. 00:00 Intro 00:45 Solvent Bottles - preventing microbial growth 07:55 Degasser - most common way people damage it & first sign of a problem. 10:08 Pump Troubleshooting - Most common problem, good chromatogram 21:40 Pump Maintenance - Replacing pump seals and plugged frit in purge valve 34:41 Autosampler Maintenance - Replacing rotor seals 47:27 Detector Tips 💡 Learn how to: ✅ Detect and fix pump seal issues ✅ Prevent microbial growth in solvent bottles ✅ Avoid damaging your degasser ✅ Replace the rotor seal in your autosampler ✅ Diagnose pressure ripple and baseline noise ✅ Handle light bulb failures in your detector This is the kind of hands-on wisdom you won’t find in textbooks — straight from someone who runs 12 HPLCs every day. 📌 Watch Part 1 -    • HPLC Demystified Part 1 | Chromatography T...   📌 Watch Part 2 -    • HPLC Demystified Part 2 | How an HPLC Syst...   🚀 Want to master HPLC with hands-on training in Chicago? Learn more at https://axionlabs.com/ #hplcchromatography #Troubleshooting #LCMaintenance #PumpSeal #RotorSeal #AnalyticalChemistry #ChromatographyTips #AxionLabs