Patch Clamp Explained (Cell-Attached, Whole Cell, Inside Out, Outside Out Configurations) | Clip

Welcome to Science With Tal! In this clip of the Signal Propagation in the Neuron video, we will discuss the different configurations of the patch clamp (cell-attached, whole cell, inside out, outside out configurations). We will then use the outside out configuration on a leak ionic channel to see how the technique can help us. To improve the quality of my content, I highly value the feedback from the viewer so do not hesitate to give any feedback in the comment section. Timestamps 0:00 Introduction 0:17 Motivation for the patch clamp 0:38 Cell attached patch clamp 1:55 Inside out patch clamp 2:15 Whole cell patch clamp 2:34 Outside out patch clamp 3:00 Summary of 4 configurations 3:11 IV curve from patch clamp (Leak potassium channel) 5:38 Word on voltage gated channels 6:47 Conclusion Resources used Here is a list of the resources that I’ve used to produce this video. (Author: title resource) Dale Purves: Neuroscience (6th edition) Eric Kandel: Principles of neural science (6th edition) Alan Hodgkin & Andrew Huxley: A quantitative description of membrane current and its application to conduction and excitation in nerve Alan Hodgkin & William Albert Hugh Rushton: The electrical constants of a crustacean nerve fibre. To have more information on these resources, you can refer to the conclusion section where a more formal citation is provided. Video credits Writing: Tal Klimenko Voice: Tal Klimenko Animations: Tal Klimenko Drawings: Tal Klimenko Editing: Tal Klimenko Introductory jingle: Thierry Du Sablond Conclusion music: lukrembo - jay (   • lukrembo - jay  )