Fluorescence Filters: Imaging Multiple Fluorophores & Dealing with Bleed-through
*This video walks you through best practices for imaging more than one fluorophore, including critical controls experiments, why bleed-through can occur when imaging multiple fluorophores and various strategies for reducing bleed-through. Enjoy! Visit FPbase.org/microscopes to generate a custom spectraviewer for your microscope. Visit Image.sc for expert help with calculating bleedthrough coefficient and image arithmetic. Visit Forum.microlist.org for expert help on the microscopy and sample preparation methods discussed in this Microcourse. Jost & Waters, 2019: https://doi.org/10.1083/jcb.201812109 French subtitles were provided by BioImaging North America.
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