[TALK 6] Fluorescent labelling & High-Resolution Microscopy - Tomás Pais de Azevedo & Sarah Lecinski

Fluorescent labelling and Super-Resolution Microscopy Speakers: Tomás Pais de Azevedo, MRC Laboratory of Molecular Biology, UK; Sarah Lecinski, MRC Laboratory of Molecular Biology, UK The LMB Light Microscopy Facility supports a diverse range of light microscopy imaging techniques within the LMB including single molecule fluorescence applications, high content screening, confocal microscopy, and intravital imaging using multiphoton excitation. In addition to commercial systems, the facility is building new instruments such as a light sheet microscope. The facility is primarily intended for hands-on research by students, postdocs, and group leaders, who prepare specimens, and operate the microscopes. Light Microscopy supports users in advice, training, assistance in imaging processing, and through the development of custom analysis software tailored to a specific application. This video gives an overview of two topics. In the first half, Tomás outlines different strategies for labelling for fluorescence microscopy. The in second, half Sarah demonstrates how different super-resolution microscopy techniques overcome the resolution limit, including: Structure Illumination Microscopy (SIM); Stimulate Emission Depletion Microscopy (STED) and Location Microscopy methods such as STORM and PALM. The Light Microscopy Facility is headed by Nick Barry, an optical physicist and specialist in light microscopy. Read more about the facility here: https://mrclmb.ac.uk/facilities/light... You can find more videos from the LMB 2025/26 Solving Problems with Molecular Techniques series here: -- About the MRC Laboratory of Molecular Biology (LMB): The LMB is one of the world's leading research institutes. Discoveries and inventions developed at the LMB, for example DNA sequencing and methods to determine the structure of proteins, have revolutionised all areas of biology. Our scientists work to advance understanding of biological processes at the molecular level. This information will help us to understand the workings of complex systems, such as the immune system and the brain, and solve key problems in human health. More links: Official Site: https://mrclmb.ac.uk/ Facebook:   / mrc.lmb   Bluesky: https://bsky.app/profile/mrclmb.bsky.... Instagram:   / mrc_lmb   LinkedIn:   / mrc-laboratory-of-molecular-biology   Click here to subscribe to MRC Laboratory of Molecular Biology on YouTube: https://www.youtube.com/user/LMBCambr...