Bioinformatics Analysis of methicilin-resistant Bacterial strain using nanopore data

Bacterial Genome Analysis of a Methicillin-Resistant Staphylococcus aureus using Nanopore Data (ONT) Download the Script and Ebook here:   / 84949857   Buy Me a Coffee: https://www.buymeacoffee.com/informat... Consultation(Video Conferencing): https://calendly.com/bioinformaticscoach Teaching(Video Conferencing): https://calendly.com/bioinformaticscoach Consultation(Audio Call): https://clarity.fm/vincentappiah Get more bioinformatics tutorials on Patreon:   / bigdataanalytics   Support my work https://www.buymeacoffee.com/informat...   / bigdataanalytics   Subscribe to my channels Bioinformatics:    / @bioinformaticscoach   Data Science:    / @datasciencecoach   Short Clips:    / @bioinformaticscforbeginners   Reach out [email protected] How to Install conda:    • How I install and configure Anaconda in  a...   How to Install Mamba :    • Install , Configure  and Run Mamba & Conda...   Original tutorial:https://training.galaxyproject.org/tr... Data download link:https://zenodo.org/record/4534098/fil... Table to GFF Script: https://github.com/vappiah/bioinfoscr... How to Install MAMBA/Micromamba :    • Install , Configure  and Run Mamba & Conda...   Install Jbrowse(Full Installation):    • JBrowse Installation and Usage for Genome ...   staramr tutorial:   • STARAMR Linux Install and Usage for Antimi...   Whole Genome Sequence Analysis | Bacterial Genome Analysis | Bioinformatics for Beginners A bioinformatics tutorial (step-by-step) on how to analyze bacterial genome sequence data. Whole Genome Sequence Analysis | Bacterial Genome Analysis of Staphylococcus aureus using Nanopore Whole Genome Sequence Analysis | Bacterial Genome Analysis | Staphylococcus Aureus Chapters 00:00 Intro 00:07 Where to get the script and ebook 01:58 Activities to be performed 02:30 PC Requirement 03:27 Installing tools using mamba or micromamba(all but jbrowse) 07:08 Create a working environment and cd into it 07:42 Download example data 09:49 Decompress the file using bzip 11:34 Quality Control 12:38 Quality Assessement of the raw_reads using NanoPlot 18:32 Filtering of Long reads using filtlong 21:08 Quality Assessement of filtered reads using NanoPlot 30:15 Genome Assembly of Long Reads(ONT) using Flye 38:03 Visualize the Genome Assemblies using Bandage 49:15 Quality Control (Evaluation) of Genome Assemblies using QUAST 51:07 QUAST output 01:00:07 Identification of Antimicrobial Resistance Genes using STARAMR 01:07:03 STARAMR Output 01:13:18 Genome Assembly Annotation with PROKKA 01:16:06 Exploring the PROKKA Outputs 01:21:42 How to Filter staramr result 01:26:08 Convert the Filtered STARAMR result Table to a GFF file 01:30:15 Mapping Long Reads(ONT) with Minimap2 01:36:27 Visualize the Result using JBROWSE bioinformatics for beginners bioinformatics tutorial bioinformatics course Bioinformatics Project #bioinformaticsforbeginners #bioinformatics #bioinformática #linuxforbeginners #linux