7) Ágar Salmonella Shigella (SS) | Meio de Cultura Seletivo | Concurso Público Microbiologia

In this lesson, we will explain in detail about Salmonella-Shigella Agar (SS), a Selective and Differential Culture Medium. Basic and Clinical Microbiology Course for Public Service Exams. Commented Questions from Public Service Exams | Biomedical Scientists, Biologists, Pharmacists, Clinical Analysis Technicians, etc. *** https://bit.ly/passaporte-ibapflix - click on the link to learn how to access more than 50 CERTIFIED courses in the health field. Take as many courses as you want with a single annual subscription. Improve your resume and qualify for the job market with the best teachers! *** 2.3 Salmonella-Shigella Agar (SS) 2.3.1 Principle SS agar has components (bile salts, brilliant green, and sodium citrate) that inhibit Gram-positive microorganisms. • The incorporation of lactose into the medium allows differentiation between lactose-positive microorganisms (bacteria that ferment lactose produce acid which, in the presence of the neutral red indicator, results in the formation of pink colonies) and bacteria that do not ferment lactose, which form transparent colonies. Sodium thiosulfate and ferric citrate allow the detection of H2S, evidenced by the formation of black colonies in the center. 2.3.2 Usefulness To select and isolate Salmonella and Shigella species in stool, food, and water samples. 2.3.3 Formula/Product Commercial medium: SS Agar. 2.3.4 Procedures Weigh and hydrate the medium according to the manufacturer's instructions. Heat the medium until the agar melts. Do not autoclave – This medium does not need to be sterilized. Cool to 50°C and distribute 20 to 25 mL into sterile 90 mm Petri dishes.  Leave at room temperature until cool; pH: 7.0 +/- 0.2 (25°C). Wrap the plates with transparent PVC plastic and store in a refrigerator at 4 to 8°C. 2.3.5 Quality control Positive: Salmonella typhimurium ATCC 14028. Negative: Staphylococcus aureus ATCC 25923. 2.3.6 Inoculation Inoculate the plates and incubate for 18 to 24 hours. If negative after 24 hours, re-incubate for another 24 hours. 2.3.7 Interpretation Original color of the medium: orange-red. • Colonies with a black center (H²S) or colorless colonies: suspected Salmonella. Colorless colonies: suspected Shigella spp. Pink or red colonies: suspected Escherichia coli or Klebsiella spp. Non-lactose fermenting bacteria are colorless. Lactose fermenting bacteria appear pink. 2.3.8 Storage and shelf life Store packaged at 4 to 8°C for 1 month. 2.3.9 Recommendations Absence of growth or scarce growth, re-incubate the plate for another 24 hours. Do not autoclave, as the high temperature degrades the sugar contained in the medium. (Source: ANVISA) *** I've selected other videos that you might also like: • Playlist with videos from the Basic and Clinical Human Microbiology Course:    • Tipos de Meios de Cultura Empregados na Mi...   • Urine culture test: how to collect urine? | Urine culture: what is the preparation?    • Exame de urocultura (cultura de urina): co...   • High leukocytes in urine: what does it mean? | And with a negative urine culture (absence of bacteria)?    • Leucócitos altos na urina o que significa?...   • Types of Culture Media Used in Clinical Microbiology | Culture of Bacteria and Fungi:    • Tipos de Meios de Cultura Empregados na Mi...   Stuart Transport Medium | Microbiology | Culture Medium | Public Service Exam Questions:    • 1) Meio de Transporte Stuart | Microbiolog...   Cary Blair Medium | Stool Transport | Stool Culture | Microbiology | Public Service Exam Questions:    • 2) Meio Cary Blair | Transporte de Fezes |...   Amies Culture Medium with Charcoal | Transport Medium | Microbiology | Public Service Exam Questions:    • 3) Meio de Cultura Amies Com Carvão | Meio...   6) Nutrient Agar | Culture Medium | Transport and Preservation | Microbiology | Public Service Exam:    • 6) Ágar Nutriente | Meio de Cultura | Tran...   7) Salmonella Shigella (SS) Agar | Selective Culture Medium | Microbiology Public Service Exam:    • 7) Ágar Salmonella Shigella (SS) | Meio de...   *** Do you want to qualify for the job market? Get ahead of the competition? The best courses and events in the biomedical field? Then you're in the right place! *** Website: https://ibapcursos.com.br/tipo-curso/... Instagram:   / ibapcursos   Facebook:   / ibapcursos