Passaging a 2 mL hPSC Suspension Culture in a 6-Well Plate
STEMCELL’s portfolio of TeSR™ 3D-optimized media products have been developed for robust and scalable suspension culture of human pluripotent stem cells (hPSCs) in aggregates. The workflows include non-enzymatic dissociation using the Gentle Cell Dissociation Reagent and filter-based trituration. Watch this technical video to learn how to passage a 2 mL hPSC suspension culture in a 6-well plate. Timecode: 0:00 Intro 0:26 Preparing required reagents and materials 1:42 Clump passaging a 3D hPSC culture using GCDR and filter-based trituration 5:09 Seeding hPSC clumps into a 6-well plate For a full list of products, as well as educational resources, visit our website: https://www.stemcell.com Follow us on Twitter: https://bit.ly/3jW20Gv Follow us on LinkedIn: https://bit.ly/32uKovJ Follow us on Instagram: https://bit.ly/3LrNkOs Like us on Facebook: https://bit.ly/32ern07 Subscribe to our YouTube channel: https://bit.ly/32f28ei
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