Proteomics Sample Preparation: Chromogenic Endotoxin Quant Kit

Endotoxin contamination is a major concern in proteomics sample preparation, especially when working with recombinant proteins, antibodies, or nucleic acids purified from Gram-negative bacteria. The *Thermo Scientific Pierce Chromogenic Endotoxin Quant Kit* provides a fast, reliable way to detect and quantify endotoxins in your samples before downstream mass spectrometry analysis. In this video, we walk through the step-by-step workflow: Preparing pyrogen-free consumables and equilibrating reagents. Setting up standards, blanks, and samples for the assay. Running the chromogenic reaction with Amebocyte Lysate (LAL). Measuring absorbance at 405 nm and generating a standard curve. Interpreting results across two sensitivity ranges (0.01–0.1 EU/mL and 0.1–1.0 EU/mL). This kit is highly specific for endotoxins, with no interference from beta-glucans, and is designed to support consistent results for protein digestion, peptide clean-up, and other MS sample prep workflows. 🔗 Learn more: https://www.thermofisher.com/us/en/ho... 📌 Chapters: 00:00 – Introduction: Why Endotoxin Detection Matters 00:50 – Preparing Standards and Samples 02:10 – Adding Amebocyte Lysate (LAL) Reagent 03:20 – Running the Chromogenic Reaction 04:40 – Measuring Absorbance and Standard Curve Setup 06:00 – Sensitivity Ranges and Data Interpretation 07:15 – Applications in Proteomics and Mass Spec Sample Prep Related Videos: Zeba Spin Desalting Columns →    • Watch how the Zeba Spin Desalting Columns ...   Purify Molecular Grade Plasmid DNA →    • Plasmid DNA Purification: Quick Guide to M...   Protein Purification Workflows (NuPAGE Novex) →    • NuPAGE Novex Bis-Tris Gel Electrophoresis ...