Spectrophotometer Calibration with Holmium Oxide Filter

http://www.fireflysci.com/wavelength-... Learn how to perform a spectrophotometer calibration with a holmium oxide filter. Spectrophotometer calibration can be tricky when not done with proper guidance. In this video we go over step by step directions on performing a spectrophotometer calibration using a holmium oxide filter. A holmium oxide filter is made specifically for wavelength calibrations. In this type of spectrophotometer calibration, you scan a large range of values and see where the peaks are. The peaks tell you in the machine is working properly. Step 1: Warm up the spectrophotometer. Step 2. Set your scan range (200-700nm for holmium oxide filter). Step 3: Perform a baseline scan. Step 4: Insert the WAV-1 UV/VIS calibration filter. Step 5: Run the scan. Step 6: Compare the data from the machine against the data from the Certificate of Calibration. If you have any questions please leave them in the comments section below or send us an e-mail at [email protected]. Here's to your success! The FireflySci Team