Reverse Transcriptase PCR or RT-PCR

Reverse transcription polymerase chain reaction (RT-PCR) is a powerful molecular biology technique used to detect and analyse RNA molecules by converting RNA into complementary DNA (cDNA), followed by PCR amplification. In this video, I explain: • How reverse transcription converts RNA into cDNA • The formation of the RNA–DNA hybrid during cDNA synthesis • How cDNA is amplified during PCR • The difference between one-step and two-step RT-PCR • Primer which may be used for reverse transcription (gene-specific, oligo(dT), and random primers) If you find this video useful, please like and subscribe. It costs you nothing and helps support the channel. Thank you! Also, if you require more information on PCR and real-time PCR, look up the videos on PCR and real-time PCR or qPCR on this channel.