Manual model building with UCSF Chimera & Coot 0.9 (Virtual workshop held May 14th, 2020)

This is a recording of a virtual workshop on manual building/refinement of atomic models in to cryoEM density maps, using UCSF Chimera for initial fitting and COOT 0.9 for building/refinement. Click "Show More" below for PDF, data and timestamps! The pdf used in the tutorial (which includes a link to all the required data) can be downloaded here: https://www.dropbox.com/s/hrsi2ntatwh... The micrographs used to generate the map are from a beautiful cryoEM dataset of hemoglobin available in EMPIAR, data generated by Mark Herzik, Mengyu Wu, and Gabe Lander: https://www.ebi.ac.uk/pdbe/emdb/empia... Useful timestamps: 2:18 - Start of chimera 5:49 - Start rigid body fit 10:09 - Inspection of model fit 12:50 - Start COOT 16:57 - Start build helix 25:29 - Renumber helix 26:11 - Merge helix 27:09 - Assign sequence of helix 30:03 - Extend helix 31:09 - Secondary structure prediction 33:13 - Generating a threaded model 35:48 - Incorporate threaded model in COOT 39:29 - Chain refine 44:17 - Loop fitting 51:48 - Heme fitting 57:15 - Comparing with reference model 58:11 - Map postprocessing comparison 1:02:51 - COOT Scripting 1:05:03 - Q&A Thanks for watching, hope it's helpful! Any/all feedback welcome :-) Cheers Oli