How to Isolate DNA Bands After Getting Double Bands with PCR? #Tutorial

In this tutorial, I covered when to use low gelling temp. agarose, and how to use it. Sometimes, with primers not working perfectly, you need to perform PCRs at lower temperatures. That brings the risk of primer attaching to multiple locations on the DNA and showing multiple bands after gel electrophoresis. In cases like this, you need to isolate the right DNA band. In this video, I covered how to isolate DNA bands using low-gelling temperature agarose. If you have any questions, please leave a comment.