Effective Strategies for Endotoxin Removal in Therapeutic Protein Purification

Endotoxins are common pyrogenic impurities in therapeutic protein preparations that make the next steps in the purification process very difficult. They are negatively charged complex aggregates of lipopolysaccharides released in large amounts from the outer membrane of gram-negative bacteria during mechanical cell lysis and death. Their presence in even minute concentrations in therapeutic protein preparations can cause septic shock, tissue injury, and even death.In research samples, endotoxins can hinder accurate data screening and immunological readouts. Endotoxins are extremely thermally stable and insensitive to pH changes, making their removal especially challenging. This webcast will present case studies using various resins that have been successfully used for endotoxin removal. Key Takeaways: • Ensuring low endotoxin levels in any downstream process. • Overcoming purification challenges. • Proven purification workflows that significantly reduce endotoxin levels for a variety of protein therapeutics. #pharmaceutical #biopharma #chromatography --- Presenter: Heidi Jones, MBA Market Development Manager, Chromatography, Bio-Rad Laboratories Heidi Jones is the market development manager for chromatography, applications & collaborations at Bio-Rad Laboratories. Over the course of 17 years at Amgen and BioMarin prior to joining Bio-Rad, Heidi specialized in protein purification as a pilot and bench scale technical scientist for a wide variety of proteins including monoclonal antibodies, bi-specifics, fc-fusions, peptides, and enzymes. Heidi holds a master’s degree in business administration from Saint Mary’s College of Moraga, California and a bachelor's degree in biology from the University of California, Los Angeles. --- Connect with Bio-Rad Online: Visit Bio-Rad's Website → http://www.bio-rad.com/ Follow Bio-Rad on LinkedIn →   / bio-rad   Follow Bio-Rad on Instagram →   / bioradlabs   Follow Bio-Rad on Facebook →   / biorad   Subscribe to Bio-Rad for the latest breakthroughs in life science research and clinical diagnostics. ©2024 Bio-Rad is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. All trademarks used herein are the property of their respective owner.