How to do a Kirby-Bauer Disk Diffusion Antimicrobial Susceptibility Test

To learn more about Hardy Diagnostics and our products visit our new website. https://hubs.la/Q04fVXy80 The purpose of the Kirby-Bauer disk diffusion susceptibility test is to determine the in vitro susceptibility of bacteria and fungi to various antimicrobial compounds. It is a standardized test that can be performed in a laboratory setting and is a valuable tool for both clinicians and microbiologists. The main benefits of this test are the flexibility of drug selection and obtaining overnight results. It is used to help guide the selection of antimicrobials for the appropriate treatment of infections caused by bacteria and fungi. The test is performed by placing antimicrobial impregnated filter paper disks on the surface of an agar-containing medium that has been inoculated with the patient’s microorganism. Upon contact with the agar surface, the antimicrobial agent immediately diffuses from the disk into the surrounding agar where it may inhibit the growth of the microorganism. If the microorganism is inhibited by the antimicrobial agent, a clear zone of ‘no growth’ will be present around the paper disk after incubation. The presence or absence of a zone of inhibition around the disks is a measure of the microorganism’s susceptibility to the antimicrobial agent. The Kirby-Bauer disk diffusion test is a standardized method for determining the susceptibility of bacteria and fungi to various antimicrobial compounds. It is a valuable tool for clinicians and microbiologists and can be used to help guide the selection of antimicrobials for the treatment of bacterial infections. By following these steps and referencing the relevant standards for current procedures, recommendations, and interpretive criteria, you can ensure that your Kirby-Bauer disk diffusion tests are accurate and reliable. To find the products listed in our video view the links below: Mueller Hinton Agar, for susceptibility testing (Kirby-Bauer) Cat. no. G45 https://hardydiagnostics.com/g45 Mueller Hinton Agar (MHA), for Kirby Bauer susceptibility testing Cat. no. H11 https://hardydiagnostics.com/h11 Blood Agar Plate, 5% Sheep Blood in Tryptic Soy Agar (TSA) Base Cat. no. A10 https://hardydiagnostics.com/a10 ReadyRack™ Tryptic Soy Broth (TSB), USP, 10ml, Glass Tube Cat. no. K82BX https://hardydiagnostics.com/k82bx McFarland Latex Standard # 0.5 Cat. no. ML05 https://hardydiagnostics.com/ml05 Wickerham Card, for Turbidity Adjustments to a McFarland Standard, 2x3 inches Cat. no. Z08 https://hardydiagnostics.com/z08 ANTIMICROBIAL DISKS https://hardydiagnostics.com/catalogs... Here are our socials: Follow us on LinkedIn   / hardydiagnostics   Follow us on Facebook:   / hardydiagnostics   About Hardy Diagnostics: We are 100% employee owned and have been serving microbiologists for over 40 years. To learn more about Hardy Diagnostics and the products we offer, visit us at https://hardydiagnostics.com/ You can also see our complete list of catalogs in the link below: https://hubs.la/Q04fVY9r0 Contact our Technical Service Team with any questions: https://hubs.la/Q04dYBrw0 Filmed and Edited By Brian Tom 00:00 History 00:22 Benefits 00:41 Overview 01:40 Susceptibility test interpretive criteria 03:58 Materials needed 04:40 Procedure starts here 05:24 McFarland standard 06:49 Streak Plate 07:41 Place disks 09:02 Incubation 09:31 View results 10:07 Measuring 10:48 Refer to breakpoint tables 11:06 Conclusion 11:45 About us Tags: kirby-bauer method of antibiotic sensitivity testing, antimicrobial susceptibility testing, kirby bauer disk diffusion method, antimicrobial susceptibility testing microbiology,antibiotic susceptibility testing,antibiotic sensitivity test, antibiotic susceptibility testing microbiology, antimicrobial susceptibility test, disk diffusion method microbiology, susceptibility test, zone of inhibition test,antibiotic susceptibility testing by disk diffusion, disc diffusion method