Phylogenetics Tutorial - Maximum Likelihood Analysis with MEGA
NOTE: I use MEGA-X in this tutorial! This video walks you through the third part of phylogenetic analysis using Sanger Sequencing products for small amplicon outputs (such as CO1, H3, 28S, etc.). This workflow is not applicable for large genomic outputs. Timestamps: 0:00 - Align & assess gene sequences 1:59 - Substitution model selection 5:56 - Running a ML Phylogeny (without Bootstrapping) 8:59 - Running a ML Phylogeny (with Bootstrapping) 10:34 - Assessing the output tree 14:03 - Exporting your tree Note: This workflow is self-taught, and it is very possible I have done something wrong. If I have, please let me know! However, this process has worked for me so far! The brands/programs I use are listed below: (Proceed at your own risk. This video is not sponsored or supported by these companies or any of their affiliates.) MEGA: megasoftware.net BEAST: beast.community/index.html (BEAUTi, BEAST, & TreeAnnotator are all included in the BEAST download package) FigTree: github.com/rambaut/figtree/releases (download the .zip file!) Check out my tutorial on using BEAUTi, BEAST, and FigTree here: • Phylogenetics Tutorial - Bayesian Analysis...

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