Plasmid and Phagemid cloning vector - Selectable vs. Screening marker
References/Resources: / the_crux This video discusses the general biology of cloning vectors focusing on plasmids and phagemids. We discuss how plasmids replicate, their general properties, diversity, origins, and many other important details. This will also help you understand the evolution of cloning vectors from a standard Plasmid to Phagemid. This video also provides you with a conceptual framework of selectable and screening markers, discussed with examples. Jump to your favorite section: 00:00 Outline 00:59 Natural plasmids 04:26 Plasmid size 06:26 ColE1 plasmid replication 10:40 pBR322 plasmid 12:36 pUC18/19 plasmid 14:23 Phagemid (pBlueScript) 17:26 Selectable markers 20:08 Screening markers 23:32 Blue-White screening Patreon Page: / the_crux Suggested videos: (M13 RCA) Rolling Circle Amplification: • Rolling Circle DNA Replication and Amplifi... Mitochondrial DNA Replication: • Mitochondrial DNA (mtDNA) Replication - D ... Lac Operon: • Lactose (Lac) Operon: an inducible operon ... Must watch playlists All videos on Genetic Engineering: • Genetic Engineering (Genes to Genomes) All videos on DNA Replication: • All about DNA Replication! All videos on Transcription: • All about Transcription! All videos on Translation: • All about Translation! Consider supporting the growth of this channel by becoming a Patron: / the_crux I appreciate your support :)
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