How to perform a CRISPR Knockout Experiment
Due to CRISPR's unparalleled ease-of-use and affordability, gene knockout experiments are now more feasible than ever before! In this video, we will show you how we used the CRISPR Cas9 system to knockout the LIF gene in a colon carcinoma cell line. This is accomplished by the delivery of a Cas9 and sgRNA lentivector into the cell line followed by drug selection and monoclonal isolation. More importantly, we demonstrate how to characterize and verify your CRISPR Knockout using the PCR-based Surveyor Assay, Sanger Sequencing, and Next Generation Sequencing. This complete process of validation ensures that your final CRISPR Knockout cell line is 100% successful. At the end of the video, we will introduce you to abm’s entire suite of CRISPR Knockout services – designed to help you jumpstart your Knockout experiments! Read our complete CRISPR Knockout Case Study here: ➜ https://info.abmgood.com/crispr-cas9-... Want more tips, tricks, guides, and protocols on how to knockout a gene using CRISPR? Join our free, 4-week #CRISPRCrashCourse: http://info.abmgood.com/crispr-crash-... Read more about the basics of the CRISPR system on our knowledge base: ➜ https://info.abmgood.com/crispr-cas9 Learn more about our CRISPR Knockout Cell Line Generation Service here: ➜ https://goo.gl/EpEZDB Watch more videos about CRISPR: ➜CRISPR Cas9 - Gene Regulation with dCas9: https://goo.gl/tRkHWd ➜ How a mini CRISPR Cas9 system is revolutionizing gene therapy! https://goo.gl/njRPv2 Thank you to all of the amazing community contributions towards translating this video into other languages including: ➜ Spanish (To view the translations, toggle the CC button and then go to Settings ➜ Subtitles/CC to select a language.) Connect with us on our social media pages to stay up to date with the latest scientific discoveries: ➜ Facebook: https://goo.gl/hc9KrG ➜ Twitter: https://goo.gl/gGGtT9 ➜ LinkedIn: https://goo.gl/kSmbht ➜ Google+: https://goo.gl/5bRNwC
How to perform a CRISPR Knockin Experiment
Gene Silencing Methods: CRISPR vs. TALENs vs. RNAi
CRISPR-Cas9 Genome Editing Technology
A step-by-step workflow for a knock-out experiment in iPSCs
The hidden logic behind #, @, & and §
If Prime Numbers Become Increasingly Rare, Then Why Do They Keep Showing Up In Pairs?
How AI Cracked the Protein Folding Code and Won a Nobel Prize
Understanding CRISPR-Cas9
Getting started with CRISPR: a review of gene knockout and homology-directed repair
But what is CRISPR-Cas9? An animated introduction to Gene Editing. #some2
How CRISPR lets us edit our DNA | Jennifer Doudna
How (and why) to take a logarithm of an image
Biologist Explains One Concept in 5 Levels of Difficulty - CRISPR | WIRED
Jennifer Doudna (UC Berkeley / HHMI): Genome Engineering with CRISPR-Cas9
5) CRISPR Cas9 - Screening and Validation Strategies
CRISPR Gene Editing: Using CRISPR-Cas9 with the Out of the Blue CRISPR Kit
Genome Editing with CRISPR-Cas9
Constructing Plasmids for Cas9/gRNA Expression and Knock Out Experiments
Essential Guide to Becoming a CRISPR Cas9 Expert - #ResearchersAtWork Webinar Series
