Séquençage de Sanger | Biochimie Facile

Hello everyone and welcome to the Easy Biochemistry channel! In this video, we explore Sanger sequencing, a key method for decoding DNA sequences. Developed in 1977 by Frederick Sanger and his team, this technique is fundamental in genetics. The process begins with chain-terminating PCR, where the target DNA is amplified. In this particular version of PCR, radiolabeled dideoxynucleotides (ddNTPs) are added, causing the elongation reaction to randomly stop, thus labeling the generated DNA fragments. Four PCR reactions are performed, each with a different type of ddNTP. The resulting fragments are then separated by electrophoresis according to their size. Finally, gel analysis reveals the DNA sequence. Each well corresponds to a specific ddNTP, and the sequence is read from the bottom to the top of the gel. For example, if the first labeled fragment is ddGTP, this means the first nucleotide in the sequence is G. We also discussed automated Sanger sequencing, where fluorophores are used instead of radiolabels. This modern method allows for more efficient and accurate DNA sequencing. If you enjoyed the video, please like, share, and comment so it can help more people ❤ Thank you! Other videos you might find interesting: Agarose Gel Electrophoresis    • ELECTROPHORESE SUR GEL D'AGAROSE | Séparat...   Polyacrylamide Gel Electrophoresis    • Séparation des protéines par ÉLECTROPHORÈS...   Specific Protein Detection by Western Blot    • Détection des protéines spécifiques par WE...   Follow me on Instagram:   / biochimie_facile   See you soon!