TotalStain Q for Total Protein Normalization | Azure Biosystems

Presented by Joy DeTorres, Application Scientist: Total protein normalization is the new standard required by journals for western blot quantification. Housekeeping proteins (HKPs) are widely used as an internal reference for western blot normalization. They are often highly expressed, lose proportionality at high protein loads, and only represent one portion of your whole sample. For this reason, using HKPs is an unreliable method of calculating normalization ratios. TotalStain Q accurately accounts for lane-to-lane variations inherent in western blotting. With linearity up to 50 μg of lysate, TotalStain Q is ideal for the normalization of lowly expressed proteins that require high sample loads. Kits are available for both PVDF and nitrocellulose membranes. TotalStain Q is compatible with any instrument capable of green channel excitation (including the Azure Imager & Sapphire Q modules). Combine TotalStain Q with your downstream near-infrared or chemiluminescent imaging protocol for quantitative analysis, without any destaining step required. This two component, room temperature stable fluorescent stain makes total protein normalization both easy and efficient.