Cell-free protein expression & the biochemistry of the PURE expression system

I don't have text for you today - I just really wanted to geek out about cell-free protein expression and especially the PURE (Protein synthesis Using Reconstituted Elements) system. So here’s an overview of cell-free expression (lysates vs. PURE etc.) and a deep dive into the journal article behind the PURE system. here are a few links and I put figures on my blog https://bit.ly/cellfreeexpression here's that key paper: Shimizu, Y., Inoue, A., Tomari, Y. et al. Cell-free translation reconstituted with purified components. Nat Biotechnol 19, 751–755 (2001). https://doi.org/10.1038/90802 And the corresponding News & Views: Swartz, J. A PURE approach to constructive biology. Nat Biotechnol 19, 732–733 (2001). https://doi.org/10.1038/90773 And that link to the RF paper: Graf, M., Huter, P., Maracci, C. et al. Visualization of translation termination intermediates trapped by the Apidaecin 137 peptide during RF3-mediated recycling of RF1. Nat Commun 9, 3053 (2018). https://doi.org/10.1038/s41467-018-05... more on translation: https://bit.ly/proteintranslationwedding &    • Protein translation - it's an amino acid w...   more on His-tag purification: http://bit.ly/histagpurification ;    • His-Tag Protein Purification theory & prac...   more on Hildegard Lamfrom: http://bit.ly/hildegardlamfrom more on creatine: http://bit.ly/creatineatp ; YouTube:    • Creatine biochemistry: creatine phosphate ...         more about all sorts of things: #365DaysOfScience All (with topics listed) 👉 http://bit.ly/2OllAB0 or search blog: http://thebumblingbiochemist.com