Fundamentals of Molecular Cloning Workshop

This session was presented at the 2025 In Vitro Biology Meeting held in Norfolk, Virginia from June 7-10, 2025 and was recorded as part of the In-Person and On-Demand Limited Access Program. The session description is as follows: PlantGENE presents the foundational steps of modular cloning strategies in this workshop. Modular cloning is a popular method for vector construction due to high efficiency and ease of design. Several genetic components can be assembled in a single reaction, typically utilizing a Golden Gate method. Many standardized toolkits are now available, and users can readily select different components to create custom vectors for various applications. Understanding the fundamentals of a modular cloning strategy for vector design is essential for successful cloning experiments. In addition to introducing key concepts, the workshop will provide troubleshooting tips to improve cloning workflow. There will be ample time for audience discussion and questions. This material is based upon work supported by the National Science Foundation under Grant No. 2220889 Learn more about the SIVB Annual Meetings at https://sivb.org/meetings.