DNA Methylation Analysis Tutorial: WGBS & RRBS for Absolute Beginners

Learn DNA methylation analysis from scratch! This comprehensive tutorial covers the complete WGBS and RRBS workflow—from raw bisulfite sequencing data to methylation calls and visualization. Perfect for bioinformatics beginners. 📖 FULL WRITTEN TUTORIAL: https://ngs101.com/how-to-analyze-dna... 🧬 WHAT YOU'LL LEARN: Differences between EPIC arrays, WGBS, and RRBS technologies Setting up your bioinformatics environment with conda Quality control with FastQC and MultiQC Adapter trimming with Trim Galore Bisulfite-aware alignment using Bismark PCR duplicate removal Methylation calling and extraction Creating genome browser tracks (BigWig files) Interpreting methylation output files and QC metrics ⏱️ TIMESTAMPS: 0:00 Introduction to DNA Methylation & Bisulfite Sequencing 1:15 EPIC Array vs WGBS vs RRBS Comparison 3:05 Environment Setup & Reference Genome Preparation 4:38 Quality Control & Adapter Trimming 5:14 Alignment with Bismark 6:22 Post-Alignment QC & Deduplication 7:29 Methylation Calling & Quantification 9:18 Understanding Methylation Context Files 11:51 Summary Files & Genome Browser Visualization 12:56 Quality Control Metrics & Validation 📊 DATASET: This tutorial uses publicly available WGBS data from GEO (GSE46644). Download instructions included in the written tutorial. 🎯 WHO IS THIS FOR? Bioinformatics beginners learning epigenomics Graduate students starting methylation projects Researchers transitioning from EPIC arrays to sequencing Anyone wanting to understand bisulfite sequencing analysis 🌐 MORE RESOURCES: NGS Learning Hub: https://ngs101.com Part 1 (EPIC Array Analysis):    • DNA Methylation Analysis: EPIC Array to DM...   📚 KEY CONCEPTS: ✓ Bisulfite conversion and C-to-T conversion ✓ CpG, CHG, and CHH methylation contexts ✓ Symmetric vs asymmetric methylation ✓ Quality metrics for successful bisulfite treatment ✓ Genome browser visualization techniques 💬 QUESTIONS? Drop them in the comments! I read and respond to every question. 👍 If this tutorial helped you, please like, subscribe, and share with your colleagues! 🔔 Subscribe for more bioinformatics tutorials covering RNA-seq, ChIP-seq, ATAC-seq, and more! --- #DNAMethylation #WGBS #RRBS #Bioinformatics #Epigenomics #BisulfiteSequencing #NGS #GenomicDataAnalysis #BioinformaticsTutorial #Bismark #ComputationalBiology #EpigeneticAnalysis #SequencingDataAnalysis #NGS101 --- DISCLAIMER: This tutorial is for educational purposes. Always refer to the official documentation for the most up-to-date parameters and best practices. © 2026 NGS Learning Hub | ngs101.com